Low-Dose Radiation (LDR) Database
About
LDR DB refers to a resource of Low-Dose or Low-Dose-Rate Radiation based Next-Generation Sequencing (NGS) Database. This comprehensive database encompasses gene expression profiling and methylation analyses conducted on human primary endothelial cells and mouse tissues subjected to low-dose (100 mGy) or low-dose-rate (6 mGy/h) radiation.
LDR DB serves as a crucial repository for NGS data derived from dedicated research projects supported by research funding from the National Research Foundation of Korea government [NRF-2020M2C8A2069337]and the Korea Institutes of Radiological & Medical Sciences [50531-2023].
LDR DB users can conveniently browse and visualize the datasets through LDR DB web portal.
NGS analysis
Transcriptome
High-throughput RNA-sequencing (RNA-Seq) is a powerful tool to characterize and quantify transcriptomes, and is now widely used in biomedical research
・ mRNA sequencing (mRNA-seq)
mRNA sequencing (mRNA-seq) is a high-throughput technique used to study the expression of genes by measuring the abundance of mRNA molecules in a biological sample.
・ QuantSeq 3’ mRNA sequencing (QuantSeq)
QuantSeq is a high-throughput sequencing-based method used for the quantitative measurement of gene expression using 3’ regions of poly(A) RNA.
・ Single-cell RNA sequencing (scRNA-seq)
scRNA-seq is an analytical technique that sequences RNA molecules at the individual cell level within an organism, enabling the exploration of gene expression patterns.
Methylation
Methylation data indicates the addition of a methyl group to DNA, influencing gene activation or inactivation. These patterns vary by cell type, development, disease state, and other factors.
Publication
1. Low-Dose Radiation Affects Cardiovascular Disease Risk in Human Aortic Endothelial Cells by Altering Gene Expression under Normal and Diabetic Conditions. Int J Mol Sci. 2022 Aug 2;23(15):8577. doi: 10.3390/ijms23158577.
2. Low-Dose Radiation-Induced Transcriptomic Changes in Diabetic Aortic Endothelial Cells. Data. 2023; 8(5):92. doi: 10.3390/data8050092.
3. Identification of DNA methylation biomarkers for evaluating cardiovascular disease risk from epigenome profiles altered by low-dose ionizing radiation. Clin Epigenetics. 2024;16(1):19. doi: 10.1186/s13148-024-01630-0.